Journal: Experimental physiology

Article Title: R-type Ca 2+ channels couple to inhibitory neurotransmission to the longitudinal muscle in the guinea pig ileum

doi: 10.1113/EP086027

Figure Lengend Snippet: CdCl2, ω-CTX but not NiCl2 inhibit IJPs recorded from circular smooth muscle cells. A, IJPs were evoked by a 10 Hz, 1 s stimulus train. These IJPs were blocked by tetrodotoxin (TTX, 0.3 μM) the P2Y1 receptor antagonist, MRS2179 (10 μM) and by the SK channel blocker, apamin (0.1 μM). The stimulus artifacts have been truncated for clarity. B, Concentration inhibition curves for the N-type Ca2+ blocker ω-CTX, and CdCl2 and NiCl2. NiCl2 (up to 50 μM) did not inhibit the IJP (n=4 for each drug). C. Circular muscle IJPs evoked by 5 Hz stimulation are not affected by NiCl2 (50 μM) or NLA (100 μM). D, NiCl2 or NiCl2 plus NLA did not change the amplitude of the single stimuli IJP (n=9). Subsequent addition of apamin (0.1 μM) inhibited the IJP (n=4, *P<0.05 vs. Control).

Article Snippet: Drug concentration response curves were fit using a 4 parameter (max, min, slope, EC 50 ) non-linear logistic function in GraphPad Prism 6.0 (GraphPad Software, Inc., LaJolla, CA).

Techniques: Concentration Assay, Inhibition, Control

Journal: Biochemical and biophysical research communications

Article Title: Discovery and characterization of selective small molecule inhibitors of the mammalian mitochondrial division dynamin, DRP1

doi: 10.1016/j.bbrc.2018.03.189

Figure Lengend Snippet: Mechanism of inhibition study on compound 4 relative to GTP shows an effect on both Vmax and KM, indicating an uncompetitive mode of binding. Analogous results were obtained with other active 1H-pyrrole-2-carboxamide compounds. Best-fit values were generated with GraphPad Prism 6.0.

Article Snippet: 1C 50 values and max % inhibition were determined using 4-parameter fits with GraphPad Prism 6.0.

Techniques: Inhibition, Binding Assay, Generated